BIOMED-J 2023

The role of chimerism monitoring following HSCT

In this whitepaper Dr. Dan Hauzenberger, Medical Director of the Section for Transplantation Immunology at Karolinska University Hospital and Chief Medical Officer at Devyser explains that, monitoring for mixed chimerism in patients after HSCT has in several studies been shown to be of clinical importance. The main purpose of the assay is to verify engraftment and subsequently monitor for the presence of MRD and potentially early detection of possible relapse.

• it is important to use an assay that offers high precision and sufficient sensitivity since it is important to monitor for increased amounts of host DNA even at low levels.
• Frequent sampling is an advantage to monitor the dynamics of MC especially if the center is interested in microchimerism as a diagnostic tool for MRD detection.
• Chimerism analysis in DNA from cell-sorted samples may be preferred over total DNA to reduce noise from DNA of non-relevant blood cells.
  
  

Guide to implementing NGS - simpler, faster, better

When planning to adopt a new NGS test in the clinical laboratory, many questions and practical considerations arise. Will introducing this new test increase or decrease complexity in the lab?  Which activities will be impacted in the wet-lab and how will this affect cost-efficiency? What equipment, materials and reagents do I need to get started? How much training will be needed?

What you’ll learn

This guide addresses frequently asked questions and highlights key factors to consider when adding a new NGS test to your offering. Learn how recent advances in NGS protocols can simplify testing workflows in your lab - saving time, improving the quality of results, and increasing efficiency. You will also find links to useful resources, such as our sequence coverage calculators and NGS checklist tool.