As stated in our IFUs and handbooks, we advise against aliquoting the activated mix, because this can decrease stability and lead to suboptimal results as observed by our R&D team.

Depending on the application and demand, different sample types and DNA extraction methods have been tested and validated for our kits. Other sample types and methods might be compatible, but users must do their own validation. For the most current and complete list, please see the ‘Sample Requirements’ section in the respective handbook or IFU for each kit currently available on our website. If in doubt, please contact techsupport@devyser.com

We have tested and validated different PCR instruments depending on the specific kit. Please refer to the ‘Materials and equipment’ section in the currently available IFU or handbook on our website for the recommended instruments. Additionally, please check the respective product page for available addendums for new instruments.

Please consult our coverage calculators, which can be found on our website.

Pooling libraries of different Devyser kits must be validated by users according to their standards and processes. Please contact techsupport@devyser.com if you have questions.

Libraries generated from germline samples can be pooled prior to purification. In contrast, libraries from somatic samples should be purified separately before being pooled. For more information on how to pool and clean up somatic samples, please see our Technical Note here.

If you want to combine germline and somatic libraries, please use our Devyser Sequence Coverage Calculator to obtain the volumes to spike in from both the somatic and germline normalized library pools.

This information can be found in the respective kit IFU or handbook under the ´Illumina sequencing ´ section. Please refer to the IFUs and handbooks currently available on our website for the latest version.

The Devyser Library Clean kit is used in the purification step of our NGS kits. The number of library pool purifications that can be performed differs depending on the NGS kit as described in the ‘Instructions for use’ section of the IFU and handbook. Please note that one library pool can contain up to 96 PCR2 libraries (when using Index Plate A).

Please see the table below for the theoretical average library sizes for each kit. These values are based on the average between the lengths of the amplicons generated during PCR 1 and 2.

The recommended concentration for the purified library pool in ng/µl is indicated in the respective IFU or handbook for each kit under the ‘Instructions for use’ section. For the corresponding molar concentration, please use the following equation for calculating together with the theoretical average PCR2 amplicon size of the respective assay.

In general, the recommended minimal coverage per amplicon is 100x for SNV and 200x for CNV for all Devyser NGS assays (except FH v2, Chimerism, and Accept cfDNA). For all assays, the respective IFU or handbook converts this to a minimal coverage per sample, as per the Devyser Sequence Coverage Calculator. This online tool also assists in planning the sequencing run, namely the number of samples that fit on a given Illumina sequencing instrument and flowcell.

Clustering issues with Illumina runs are generally due to incorrect loading concentration of sequencing libraries. However, other reasons could include:

• Inaccurate library quantification – quantification should be done using a Qubit instrument and assay kit as described in the respective IFU or handbook. Fresh standards should also be prepared and measured for each preparation, and reagents must be at room temperature. Other methods such as NanoDrop and Tapestation/Bioanalyzer are not as precise as fluorometric approaches (e.g., Qubit), so we strongly advise against using them for library quantification.
• Inefficient library denaturation – always prepare freshly diluted NaOH for denaturing libraries prior to sequencing. The NaOH stock solution must have a pH >12.5 prior to any dilution.

It is important to consider that under-clustering is normally preferable to over-clustering, as it affects data quantity than quality (except on MiniSeq). If in doubt, or when first optimizing the assay, please aim for the lower end of the recommended loading concentration as stated in our IFUs and handbooks to avoid over-clustering.

The same number of analyses can be performed as tests purchased (i.e., number of reactions in a kit or kits). For example, if a 24-test kit is purchased, you can perform 24 analyses with our software.

Please ensure that you have a stable internet connection and that the FASTQ files are stored locally. Make sure the software is installed locally and not on a server. If the issue persists after one re-upload attempt, please contact techsupport@devyser.com

To request access to the Amplicon Suite software, please contact your local sales representative (please find also the list of our distributors here). Our team will contact you with installation instructions and account credentials.

You can check the version you are currently using in the ‘About’ tab in the Amplicon Suite software. If there is an update available, you will normally be prompted to update when starting the software. Also, there is always a notification in the ‘News’ tab when a new version is released. There, you can see the update details, namely the release notes.

Yes, multiple users can simultaneously access the same account with the same login credentials.

Yes. Devyser Fragment Analysis kits have been validated with GeneMapper versions 4, 5, and 6. The settings files can be found on ‘Software settings’ in the ‘Downloads’ section in the respective product page on our website. For other versions, please contact techsupport@devyser.com

There could be several causes, for further assistance please refer to our troubleshooting guide and the respective IFU.

If you experience crosstalk, please reduce the DNA input if the signals seem too high, and if the issue persists, redo spectral calibration and ensure that the calibration passes. For further assistance, please learn more in our troubleshooting guide.