Devyser CFTR NGS
Detect all mutations in one test with Devyser’s CE-IVD single tube, next-generation sequencing (NGS) library prep kit for targeted, complete CFTR gene sequencing.
Discover the advantages of Devyser’s NGS workflow
- One tube per sample means no need for sample splitting
- Reduce hands-on time from days to under 45 minutes
- Direct detection of CNVs
- Detect all mutations in the coding regions of the CFTR gene
- Determination of poly-T and TG repeats
- Choice of several validated software options, including CNV analysis
Cystic Fibrosis Molecular Diagnostics
CFTR mutation testing can be used in diagnosis of cystic fibrosis, male infertility caused by CBAVD, and acute recurrent or chronic pancreatitis. It can also be used to guide targeted therapies, and as an aid in newborn screening. More than 2,000 mutations and variants in the CFTR gene have been described. The vast majority of these mutations have a population frequency below 0.1 % with high heterogeneity of mutation distribution between different ethnic groups. Devyser’s range of diagnostic CFTR kits enables allele specific detection as well as targeted, complete, CFTR gene sequencing using NGS for detection of all mutations, known and unknown, in a single test.
Designed for routine NGS diagnostics
The Devyser CFTR kit is easy to implement and highly cost-effective, making it a good match for laboratories of any size. With ready-to-use reagents and a user-friendly workflow, it suites both manual and automated workflows. Devyser’s unique single-tube approach simplifies the workflow, reduces hands-on time and minimizes the risk of sample mix-up and contamination. The proprietary multiplex PCR primer chemistry provides full and uniform coverage of the CFTR gene.
Watch video to see how it works
Coverage includes all exons and exon/intron junctions, the promoter region and several clinically relevant deep intronic mutations. Overlapping primer design is used to ascertain superior INDEL and primer site mutation coverage as well as downstream CNV analysis. In addition, the kit also allows analysis of poly-T variants along with the upstream TG-repeat region.
CE-IVD
100%
<45 min
>95%
<5 hours
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Learn more about how our single-tube NGS assay can help your team get the results they need.
Whitepaper
NGS: Changing the Game in CFTR Analysis by Dr. Stewart Payne
Many testing strategies have been developed in laboratories across the world. Optimal strategies generally include what might be termed “level 1” and “level 2” testing. Until the recent application of next generation sequencing (NGS), no single-pass assay has been capable of routinely screening for all published CFTR mutations.
Whitepapers
NGS: Changing the Game in CFTR Analysis by Dr. Stewart Payne
This paper provides straightforward advice for laboratory professionals on how to implement NGS for routine CFTR diagnostics. Authored by Dr. Stewart Payne, Consultant medical scientist in the UK NHS, it includes useful sections on: CFTR testing strategies, level 1 and level 2 testing, NGS testing strategies, interpretation of CFTR mutation data, CFTR diagnostics and targeted therapies and much more.
Whitepaper
Next Generation Sequencing: Changing the game in CFTR analysis by Dr Stewart Payne
Until the recent application of next generation sequencing (NGS), no single-pass assay has been capable of routinely screening for all published CFTR mutations.
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Download a specific Batch Release Certificate (BRC) below by entering the BATCH/LOT number and then click on "Find".
Whitepapers
NGS: Changing the Game in CFTR Analysis by Dr. Stewart Payne
This paper provides straightforward advice for laboratory professionals on how to implement NGS for routine CFTR diagnostics. Authored by Dr. Stewart Payne, Consultant medical scientist in the UK NHS, it includes useful sections on: CFTR testing strategies, level 1 and level 2 testing, NGS testing strategies, interpretation of CFTR mutation data, CFTR diagnostics and targeted therapies and much more.
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We are specialists when it comes to diagnostic kits for complex DNA testing within oncology, reproductive health and hereditary diseases. Our products are used to guide targeted cancer therapies, to enable rapid prenatal diagnostics, as well as in a wide array of genetic tests. Routine diagnostic laboratories in more than 50 countries worldwide use our products.