Introduction to QF-PCR by Kathy Mann
This paper provides straightforward advice for laboratory professionals on how to operate a high-quality QF-PCR service — from basic set up to best practice guidelines.
Authored by Dr. Kathy Manns, the pioneer in prenatal QF-PCR analysis, it includes useful sections on: assay design, sample processing, limitations and much more.
In this white paper, you will learn about:
- Principle of QF-PCR and its advantages
- Why the availability of commercial QF-PCR assays has proven to be a welcome development
- Best practice guidelines for QF-PCR
- Factors that can lead to incidences of samples where no QF-PCR result is possible
- What the future holds for prenatal screening
Principle of QF-PCR
QF-PCR analysis exploits the variability of microsatellite sequences (STRs), that vary in length at any given locus to distinguish between chromosome homologues. The detection and quantitation of STR alleles enables relative chromosome quantitation (see Figure 1).