Aneuploidy QF-PCR

For fast, precise and cost-effective prenatal aneuploidy diagnosis, QF-PCR is the technology of choice. Devyser’s market-leading QF-PCR kits bring you these benefits, and more, in a ready-to-use kit:
  • Results in less than five hours, with less than 90 minutes hands on time, allowing your laboratory to provide results in just one day.
  • No need for cell cultures.
  • Efficient and sensitive detection of chromosomal aneuploidies and maternal cell contamination (MCC) in DNA samples prior to microarray analysis.
  • Fewer re-runs thanks to exceptional assay performance and the use of a large number of genetic markers selected to maximise cross-ethnical informativity.
  • Reliable detection of Turner syndrome through the use of unique X-chromosome counting markers.
  • Validated for use on all Thermo Fisher/ABI Capillary electrophoresis instruments
  • Easy handling. All reagents are ready to use reducing the time needed for PCR set-up and the risk of contamination. Easy results interpretation. GeneMapper and GeneMarker plugins are available for easy results interpretation and reporting.

Devyser Compact QF-PCR – one single mix, 26 genetic markers 

Devyser Compact enables rapid prenatal aneuploidy detection of chromosomes 13, 18, 21 and XY in a single PCR mix.

In addition to detecting Down, Edwards, Patau and Klinefelter syndromes, the Devyser Compact kit includes our patented technology for reliable diagnosis of Turner syndrome, using two dedicated X-chromosome counting markers. The CE-IVD certified Devyser Compact kit relies on quantitative, multiplex PCR amplification of 26 highly informative genetic markers in a single mix for rapid prenatal diagnosis of the most common autosomal and sex chromosome aneuploidies.

  • Efficient and sensitive detection of chromosomal aneuploidies and maternal cell contamination (MCC) in DNA samples prior to microarray analysis
  • No sample mix-up. One single tube per sample during PCR and detection reduces the risk of sample mix-up.
  • Quick and easy implementation. All reagents needed to perform QF-PCR are provided in a single kit.

Devyser QF-PCR Versions – Needs-Specific Analysis

Eliminate cell culture, increase analysis success rates and speed up your pregnancy loss aneuploidy analysis for chromosomes 13, 15, 16, 18, 21, 22 and XY using QF-PCR

Pregnancy loss analysis
Women who have undergone one or more spontaneous abortions caused by chromosomal abnormalities are at increased risk for chromosomal abnormalities in future pregnancies. Cytogenetic studies of miscarriages are highly recommended even in the case of the first pregnancy loss. Identification of the possible cause of fetal loss significantly reduces the long-term psychological distress associated with a miscarriage and enables improved genetic counselling in future pregnancies. The most frequently observed numerical chromosomal abnormalities involve chromosomes 13, 15, 16, 18, 21, 22 and X.

Conventional analysis
Conventional cytogenetic studies (karyotyping or FISH) are expensive and need a long period of time in order to obtain results. Moreover, they result in high rates of culture failure, misdiagnosis due to maternal contamination and cell overgrowth as well as insufficient quality of chromosome preparations. Cell culture may selectively yield normal karyotypes or selected abnormal karyotypes that survive in-vitro cell proliferation.

QF-PCR for pregnancy loss analysis
QF-PCR does not require cell culture, requires minute amounts of tissue material and allows the lab to obtain results within one working day. The Devyser Extend kit includes 42 genetic markers for aneuploidy analysis by QF-PCR of chromosomes 13, 15, 16, 18, 21, 22, X and Y. Two unique X-chromosome counting markers for reliable detection of Turner syndrome are also included in Devyser Extend.

Resolution or Reflex Testing

The Devyser Resolution kits are QF-PCR kits intended for rapid prenatal aneuploidy analysis of individual chromosomes, including chromosomes 13, 18, 21 and XY  

Devyser Resolution 21 is a QF-PCR kit intended for resolution or reflex testing of chromosome 21 aneuploidies. The kit enables increased marker resolution capabilities by combining STR markers used in the Devyser Compact and Devyser Complete kits with additional STR markers for reflex testing of chromosome 21. The kit allows analysis of totally nine (9) STR markers on chromosome 21. 

Devyser Resolution XY is a QF-PCR kit intended for resolution or reflex testing of chromosomes X and Y. The kit enables increased marker resolution capabilities by combining X and Y markers used in the Devyser Compact and Devyser Complete kits with additional markers for advanced reflex testing of chromosomes X and Y. The kit allows analysis of totally seventeen (17) genetic markers on chromosomes X and Y. 

The Devyser Resolution XY kit includes N X-chromosomal STR markers (Representing markers present on both arms), N pseudoautomal STR-markers three (3) unique X-chromosome counting markers for reliable detection of Turner syndrome and Y-chromosomal markers from both arms. 

Devyser Resolution 18 is a QF-PCR kit intended for resolution or reflex testing of chromosome 18 aneuploidies. The kit enables increased marker resolution capabilities by combining STR markers used in the Devyser Compact and Devyser Complete kits with additional STR markers for reflex testing of chromosome 18. The kit allows analysis of totally nine (9) STR markers on chromosome 18. 

Devyser Resolution 13 is a QF-PCR kit intended for resolution or reflex testing of chromosome 13 aneuploidies. The kit enables increased marker resolution capabilities by combining STR markers used in the Devyser Compact and Devyser Complete kits with additional STR markers for reflex testing of chromosome 13. The kit allows analysis of totally nine (9) STR markers on chromosome 13. 

Devyser Complete QF-PCR

Devyser Complete enables rapid prenatal aneuploidy detection of chromosomes 13, 18, 21 and XY using 33 genetic markers in two separate PCR mixesCross mix ID markers are included in both mixes to reduce the risk of sample mix-up. In addition to detecting Down, Edwards, Patau and Klinefelter syndromes, the Devyser Complete kit includes our patented technology for reliable diagnosis of Turner syndrome, using two dedicated X-chromosome counting markers. 

Aneuploidy QF-PCR

For fast, precise and cost-effective prenatal aneuploidy diagnosis, QF-PCR is the technology of choice. Devyser’s market-leading QF-PCR kits bring you these benefits, and more, in a ready-to-use kit: 

  • Results in less than five hours, with less than 90 minutes hands on time, allowing your laboratory to provide results in just one day.
  • No need for cell cultures.
  • Efficient and sensitive detection of chromosomal aneuploidies and maternal cell contamination (MCC) in DNA samples prior to microarray analysis.
  • Fewer re-runs thanks to exceptional assay performance and the use of a large number of genetic markers selected to maximise cross-ethnical informativity.
  • Reliable detection of Turner syndrome through the use of unique X-chromosome counting markers.
  • Validated for use on all Thermo Fisher/ABI Capillary electrophoresis instruments
  • Easy handling. All reagents are ready to use reducing the time needed for PCR set-up and the risk of contamination. Easy results interpretation. GeneMapper and GeneMarker plugins are available for easy results interpretation and reporting.

Devyser Compact QF-PCR – one single mix, 26 genetic markers 

Devyser Compact enables rapid prenatal aneuploidy detection of chromosomes 13, 18, 21 and XY in a single PCR mix.

In addition to detecting Down, Edwards, Patau and Klinefelter syndromes, the Devyser Compact kit includes our patented technology for reliable diagnosis of Turner syndrome, using two dedicated X-chromosome counting markers. The CE-IVD certified Devyser Compact kit relies on quantitative, multiplex PCR amplification of 26 highly informative genetic markers in a single mix for rapid prenatal diagnosis of the most common autosomal and sex chromosome aneuploidies.

  • Efficient and sensitive detection of chromosomal aneuploidies and maternal cell contamination (MCC) in DNA samples prior to microarray analysis
  • No sample mix-up. One single tube per sample during PCR and detection reduces the risk of sample mix-up.
  • Quick and easy implementation. All reagents needed to perform QF-PCR are provided in a single kit.

Devyser QF-PCR Versions – Needs-Specific Analysis

Eliminate cell culture, increase analysis success rates and speed up your pregnancy loss aneuploidy analysis for chromosomes 13, 15, 16, 18, 21, 22 and XY using QF-PCR

Pregnancy loss analysis
Women who have undergone one or more spontaneous abortions caused by chromosomal abnormalities are at increased risk for chromosomal abnormalities in future pregnancies. Cytogenetic studies of miscarriages are highly recommended even in the case of the first pregnancy loss. Identification of the possible cause of fetal loss significantly reduces the long-term psychological distress associated with a miscarriage and enables improved genetic counselling in future pregnancies. The most frequently observed numerical chromosomal abnormalities involve chromosomes 13, 15, 16, 18, 21, 22 and X.

Conventional analysis
Conventional cytogenetic studies (karyotyping or FISH) are expensive and need a long period of time in order to obtain results. Moreover, they result in high rates of culture failure, misdiagnosis due to maternal contamination and cell overgrowth as well as insufficient quality of chromosome preparations. Cell culture may selectively yield normal karyotypes or selected abnormal karyotypes that survive in-vitro cell proliferation.

QF-PCR for pregnancy loss analysis
QF-PCR does not require cell culture, requires minute amounts of tissue material and allows the lab to obtain results within one working day. The Devyser Extend kit includes 42 genetic markers for aneuploidy analysis by QF-PCR of chromosomes 13, 15, 16, 18, 21, 22, X and Y. Two unique X-chromosome counting markers for reliable detection of Turner syndrome are also included in Devyser Extend.

Resolution or Reflex Testing

The Devyser Resolution kits are QF-PCR kits intended for rapid prenatal aneuploidy analysis of individual chromosomes, including chromosomes 13, 18, 21 and XY  

Devyser Resolution 21 is a QF-PCR kit intended for resolution or reflex testing of chromosome 21 aneuploidies. The kit enables increased marker resolution capabilities by combining STR markers used in the Devyser Compact and Devyser Complete kits with additional STR markers for reflex testing of chromosome 21. The kit allows analysis of totally nine (9) STR markers on chromosome 21. 

Devyser Resolution XY is a QF-PCR kit intended for resolution or reflex testing of chromosomes X and Y. The kit enables increased marker resolution capabilities by combining X and Y markers used in the Devyser Compact and Devyser Complete kits with additional markers for advanced reflex testing of chromosomes X and Y. The kit allows analysis of totally seventeen (17) genetic markers on chromosomes X and Y. 

The Devyser Resolution XY kit includes N X-chromosomal STR markers (Representing markers present on both arms), N pseudoautomal STR-markers three (3) unique X-chromosome counting markers for reliable detection of Turner syndrome and Y-chromosomal markers from both arms. 

Devyser Resolution 18 is a QF-PCR kit intended for resolution or reflex testing of chromosome 18 aneuploidies. The kit enables increased marker resolution capabilities by combining STR markers used in the Devyser Compact and Devyser Complete kits with additional STR markers for reflex testing of chromosome 18. The kit allows analysis of totally nine (9) STR markers on chromosome 18. 

Devyser Resolution 13 is a QF-PCR kit intended for resolution or reflex testing of chromosome 13 aneuploidies. The kit enables increased marker resolution capabilities by combining STR markers used in the Devyser Compact and Devyser Complete kits with additional STR markers for reflex testing of chromosome 13. The kit allows analysis of totally nine (9) STR markers on chromosome 13. 

Devyser Complete QF-PCR

Devyser Complete enables rapid prenatal aneuploidy detection of chromosomes 13, 18, 21 and XY using 33 genetic markers in two separate PCR mixesCross mix ID markers are included in both mixes to reduce the risk of sample mix-up. In addition to detecting Down, Edwards, Patau and Klinefelter syndromes, the Devyser Complete kit includes our patented technology for reliable diagnosis of Turner syndrome, using two dedicated X-chromosome counting markers. 

  • Whitepapers
  • Downloads

Whitepapers

Whitepaper INTRODUCTION QF-PCR BY KATHY MANN picture

Introduction to QF-PCR by Kathy Mann

This paper provides straightforward advice for laboratory professionals on how to operate a high-quality QF-PCR service — from basic set up to best practice guidelines. Authored by Dr. Kathy Mann, the pioneer in prenatal QF-PCR analysis, it includes useful sections on: assay design, sample processing, limitations and much more.

Picture Whitepaper Detecting Mosaicism with QF-PCR

Detecting Mosaicism with QF-PCR by Dr. Kathy Mann

Given the significance of mosaicism to prenatal diagnosis, it is clear that any technique used in the diagnosis of aneuploidy in prenatal samples should be able to detect aneuploid mosaicism. With these criteria in mind, the use of quantitative fluorescence-PCR (QF-PCR) analysis is suitable (see Expert Review 01: An introduction to QF-PCR).

This paper provides straightforward advice for laboratory professionals on how to use QF-PCR to identify mosaicism in prenatal diagnostics. Authored by Dr. Kathy Mann, the pioneer in prenatal QF-PCR analysis, it includes useful sections on: origin, detection and incidence of mosaicism and more.

Whitepaper picture QF-PCR AND MATERNAL CELL CONTAMINATION

QF-PCR and Maternal Cell Contamination by Dr. Kathy Mann

The ability of QF-PCR to identify Maternal Cell Contamination (MCC) in uncultured Amniotic Fluid (AF) and Chorionic Villi (CV) offers a real benefit over both Fluorescence in Situ Hybridisation (FISH) analysis of interphase nuclei and other molecular methods of rapid aneuploidy diagnosis such as MLPA and BACs-on-Beads (BoBs), where MCC of samples from female pregnancies cannot be detected.

This paper offers solid advice for laboratory professionals on using a QF-PCR-based approach to identify maternal cell contamination in uncultured AF and CV samples. Authored by Dr. Kathy Mann, the pioneer in prenatal QF-PCR analysis.

Whitepaper QF-PCR IN PREGNANCY LOSS ANALYSIS picture

QF-PCR in Pregnancy Loss Analysis by Dr. Helen White

This paper provides insights for laboratory professionals on the advantages of using extended QF-PCR in the genetic analysis of pregnancy loss samples. The paper includes useful sections on: current technologies, QF-PCR analysis in routine practice, special considerations and more. Authored by Dr. Helen White, Senior Clinical Lecturer at the UK National Genetics Reference Laboratory (Wessex).

Ask Us a Question

We are specialists when it comes to diagnostic kits for complex DNA testing within oncology, reproductive health and hereditary diseases. Our products are used to guide targeted cancer therapies, to enable rapid prenatal diagnostics, as well as in a wide array of genetic tests. Routine diagnostic laboratories in more than 50 countries worldwide use our products.