Single tube Thalassemia kit

Complete NGS analysis of HBA1, HBA2 and HBB in a single run with Devyser Thalassemia

Simple, fast and accurate

Using the Devyser Thalassemia kit, we can reduce both response time and costs. 
O
ne single assay provides all the information we need.

Prof. Dr Veysel Sabri Hancer,
Istinye University Genetic Diseases Diagnostic Centre, Istanbul, Turkey 

Discover the advantages

Complete one-tube analysis

Single-tube NGS method for both SNV and CNV detection in HBA1, HBA2 and HBB

Fast and simple NGS workflow

From DNA to sequencing in less than 5 hours with less than 45 mins hands-on time

User-friendly data analysis software

Bypass complex and laborious data analysis with our tailored analytical software solution

One assay for all Thalassemia testing needs

Simple procedure to detect all mutations in HBA1, HBA2 & HBB

With the Devyser Thalassemia kit you can detect all mutations in HBA1, HBA2 and HBB using a single, one-tube NGS assay.

The assay detects single nucleotide polymorphisms (SNVs) Indels and CNVs. The extremely simple procedure makes it an excellent choice for any lab, whether you are running advanced second level genetic testing or large scale mutation screening.

The fast, simple and robust NGS workflow replaces complex multi-step protocols and eliminates the need for maintaining multiple Thalassemia assays in your lab.

Comprehensive CNV detection

Devyser’s proprietary PCR chemistry provides complete and uniform coverage of the relevant genetic regions by enabling a high level of overlapping amplicon multiplexing.

This simple workflow significantly reduces the risk for contamination and sample mix-up. Reliable detection of large structural deletion is further improved through the use of PCR primers aligned to both ends of 18 large deletions with high prevalence. Simultaneously, coverage based CNV analysis is used to confirm detected deletions and to identify additional large structural deletions in the targeted regions.

See how it’s done

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Simple NGS workflow

Only one tube per sample 

Single tube library protocol dramatically reduces the risk for sample  mix up or contamination and minimises the hands-on time 

Pre-dispensed dual indices in strip/plate 

All indexes are delivered predispensed in strip or plate format to reduce the risk for sample mix up 
or contamination and 
to minimise
the hands-on time
 

Sample pooling before cleanup and NGS

All patient samples are pooled to a single tube before clean-up, again reducing the hands-on time and simplifying the workflow 

Devyser Thalassemia detects additional variants

In 15% of samples, additional pathogenic sequence variants were found with the majority in the other globin gene. These sequence variants had previously not been identified given that current analysis workflows often stop as soon as a pathogenic sequence variants is found in one of the two gene clusters.

125 characterised clinical samples analysed with Devyser Thalassemia NGS

100% of previously characterised pathogenic variants were detected. 

15% of samples contained additional, previously undetected pathogenic variants.

Data analysis

Bypass complex and laborious data analysis with our tailored analytical software solution

Simple graphical intepretation of sequence variants and CNV's

Complete NGS thalassemia data interpretation

View poster on how it works

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IFU and software settings

Enter access code found in the lower right corner of the label on the kit box.

Certificates

Download a specific Batch Release Certificate (BRC) below by entering the BATCH/LOT number and then click on "Find".

Product details

CE-IVD Certified Kits

Name
Scope
Pack Size
Article Number
Devyser Thalassemia
"Full sequence analysis of the following genes: HBA1, HBA2, HBB Alpha-globin genecluster CNV analysis: POLR3K, HS-40, HBZ, HBZP1, HBAP1, HBA2, HBA1, HBQ1, LUC7L, AXIN1 Beta-globin gene cluster CNV analysis: LCRB, HBG2, HBG1, HBD, HBB, 3'HS1"
24
8-A106-24
48
8-A106-48

Research Use Only Kits

Name
Scope
Pack Size
Article Number
Devyser Thalassemia
"Full sequence analysis of the following genes: HBA1, HBA2, HBB Alpha-globin genecluster CNV analysis: POLR3K, HS-40, HBZ, HBZP1, HBAP1, HBA2, HBA1, HBQ1, LUC7L, AXIN1 Beta-globin gene cluster CNV analysis: LCRB, HBG2, HBG1, HBD, HBB, 3'HS1"
24
8-A106-24-RUO
48
8-A106-48-RUO

Ask Us a Question

We are specialists when it comes to diagnostic kits for complex DNA testing within oncology, reproductive health and hereditary diseases. Our products are used to guide targeted cancer therapies, to enable rapid prenatal diagnostics, as well as in a wide array of genetic tests. Routine diagnostic laboratories in more than 50 countries worldwide use our products.

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