Aneuploidy QF-PCR
For fast, precise and cost-effective prenatal aneuploidy diagnosis, QF-PCR is the technology of choice.
Devyser’s market-leading QF-PCR kits bring you these benefits, and more, in a ready-to-use kit:
- Results in less than five hours, with less than 90 minutes hands on time, allowing your laboratory to provide results in just one day.
- Rapid diagnostic follow-up after NIPT testing.
- No need for cell cultures.
- Efficient and sensitive detection of chromosomal aneuploidies and maternal cell contamination (MCC) in DNA samples prior to microarray analysis.
- Fewer re-runs thanks to exceptional assay performance and the use of a large number of genetic markers selected to maximise cross-ethnical informativity.
- Reliable detection of Turner syndrome through the use of unique X-chromosome counting markers.
- Easy handling. All reagents are ready to use reducing the time needed for PCR set-up and the risk of contamination.
- GeneMapper and GeneMarker plugins are available for easy results interpretation and reporting.
Devyser Compact: one single mix, 26 genetic markers
Devyser Compact enables rapid prenatal aneuploidy detection of chromosomes 13, 18, 21 and XY in a single PCR mix.
In addition to detecting Down, Edwards, Patau and Klinefelter syndromes, the Devyser Compact kit includes our patented technology for reliable diagnosis of Turner syndrome, using two dedicated X-chromosome counting markers. The CE-IVD certified Devyser Compact kit relies on quantitative, multiplex PCR amplification of 26 highly informative genetic markers in a single mix for rapid prenatal diagnosis of the most common autosomal and sex chromosome aneuploidies.
- Efficient and sensitive detection of chromosomal aneuploidies and maternal cell contamination (MCC) in DNA samples prior to microarray analysis
- No sample mix-up. One single tube per sample during PCR and detection reduces the risk of sample mix-up.
- Quick and easy implementation. All reagents needed to perform QF-PCR are provided in a single kit.
Devyser Extend for rapid miscarriage analysis
Eliminate cell culture, increase analysis success rates and speed up your pregnancy loss aneuploidy analysis for chromosomes 13, 15, 16, 18, 21, 22 and XY using Devyser Extend.
Pregnancy loss analysis
Women who have undergone one or more spontaneous abortions caused by chromosomal abnormalities are at increased risk for chromosomal abnormalities in future pregnancies. Cytogenetic studies of miscarriages are highly recommended even in the case of the first pregnancy loss. Identification of the possible cause of fetal loss significantly reduces the long-term psychological distress associated with a miscarriage and enables improved genetic counselling in future pregnancies. The most frequently observed numerical chromosomal abnormalities involve chromosomes 13, 15, 16, 18, 21, 22 and X.
Conventional analysis
Conventional cytogenetic studies (karyotyping or FISH) are expensive and need a long period of time in order to obtain results. Moreover, they result in high rates of culture failure, misdiagnosis due to maternal contamination and cell overgrowth as well as insufficient quality of chromosome preparations. Cell culture may selectively yield normal karyotypes or selected abnormal karyotypes that survive in-vitro cell proliferation.
QF-PCR for pregnancy loss analysis
QF-PCR does not require cell culture, requires minute amounts of tissue material and allows the lab to obtain results within one working day. The Devyser Extend kit includes 42 genetic markers for aneuploidy analysis by QF-PCR of chromosomes 13, 15, 16, 18, 21, 22, X and Y. Two unique X-chromosome counting markers for reliable detection of Turner syndrome are also included in Devyser Extend.
Resolution or Reflex Testing
The Devyser Resolution kits are QF-PCR kits intended for rapid prenatal aneuploidy analysis of individual chromosomes, including chromosomes 13, 18, 21 and XY. See product details for more information.
Devyser Complete QF-PCR
Devyser Complete enables rapid prenatal
aneuploidy detection of chromosomes 13, 18, 21 and XY using 33 genetic markers in two separate PCR mixes. Cross mix ID markers are included in both mixes to reduce the risk of sample mix-up. In addition to detecting Down, Edwards, Patau and Klinefelter syndromes, the Devyser Complete kit includes our patented technology for reliable diagnosis of Turner syndrome, using two dedicated X-chromosome counting markers.
Whitepapers
Introduction to QF-PCR by Kathy Mann
This paper provides straightforward advice for laboratory professionals on how to operate a high-quality QF-PCR service — from basic set up to best practice guidelines. Authored by Dr. Kathy Mann, the pioneer in prenatal QF-PCR analysis, it includes useful sections on: assay design, sample processing, limitations and much more.
Detecting Mosaicism with QF-PCR by Dr. Kathy Mann
Given the significance of mosaicism to prenatal diagnosis, it is clear that any technique used in the diagnosis of aneuploidy in prenatal samples should be able to detect aneuploid mosaicism. With these criteria in mind, the use of quantitative fluorescence-PCR (QF-PCR) analysis is suitable (see Expert Review 01: An introduction to QF-PCR).
This paper provides straightforward advice for laboratory professionals on how to use QF-PCR to identify mosaicism in prenatal diagnostics. Authored by Dr. Kathy Mann, the pioneer in prenatal QF-PCR analysis, it includes useful sections on: origin, detection and incidence of mosaicism and more.
QF-PCR and Maternal Cell Contamination by Dr. Kathy Mann
The ability of QF-PCR to identify Maternal Cell Contamination (MCC) in uncultured Amniotic Fluid (AF) and Chorionic Villi (CV) offers a real benefit over both Fluorescence in Situ Hybridisation (FISH) analysis of interphase nuclei and other molecular methods of rapid aneuploidy diagnosis such as MLPA and BACs-on-Beads (BoBs), where MCC of samples from female pregnancies cannot be detected.
This paper offers solid advice for laboratory professionals on using a QF-PCR-based approach to identify maternal cell contamination in uncultured AF and CV samples. Authored by Dr. Kathy Mann, the pioneer in prenatal QF-PCR analysis.
QF-PCR in Pregnancy Loss Analysis by Dr. Helen White
This paper provides insights for laboratory professionals on the advantages of using extended QF-PCR in the genetic analysis of pregnancy loss samples. The paper includes useful sections on: current technologies, QF-PCR analysis in routine practice, special considerations and more. Authored by Dr. Helen White, Senior Clinical Lecturer at the UK National Genetics Reference Laboratory (Wessex).
- Whitepapers
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IFU and software settings
Enter access code found in the lower right corner of the label on the kit box.
Certificates
Download a specific Batch Release Certificate (BRC) below by entering the BATCH/LOT number and then click on "Find".
Best practice guidelines - QF-PCR
Filesize: 0 bytesSDS - Devyser Compact v3
SDS - Devyser Extend V2
SDS - Devyser Complete v2
SDS - Devyser Resolution v2
Application Note MCC
Filesize: 0 bytesDevyser Extend v2 Flyer
Filesize: 0 bytesDevyser Compact v3 Flyer
Filesize: 0 bytesDevyser Complete v2 Flyer
European guidelines for constitutional cytogenomic analysis
Filesize: 0 bytesFlyer - Devyser Resolution
Filesize: 0 bytesProduct Information Booklet - Devyser Solutions for Prenatal QF-PCR
Filesize: 0 bytesWhitepapers
Introduction to QF-PCR by Kathy Mann
This paper provides straightforward advice for laboratory professionals on how to operate a high-quality QF-PCR service — from basic set up to best practice guidelines. Authored by Dr. Kathy Mann, the pioneer in prenatal QF-PCR analysis, it includes useful sections on: assay design, sample processing, limitations and much more.
Detecting Mosaicism with QF-PCR by Dr. Kathy Mann
Given the significance of mosaicism to prenatal diagnosis, it is clear that any technique used in the diagnosis of aneuploidy in prenatal samples should be able to detect aneuploid mosaicism. With these criteria in mind, the use of quantitative fluorescence-PCR (QF-PCR) analysis is suitable (see Expert Review 01: An introduction to QF-PCR).
This paper provides straightforward advice for laboratory professionals on how to use QF-PCR to identify mosaicism in prenatal diagnostics. Authored by Dr. Kathy Mann, the pioneer in prenatal QF-PCR analysis, it includes useful sections on: origin, detection and incidence of mosaicism and more.
QF-PCR and Maternal Cell Contamination by Dr. Kathy Mann
The ability of QF-PCR to identify Maternal Cell Contamination (MCC) in uncultured Amniotic Fluid (AF) and Chorionic Villi (CV) offers a real benefit over both Fluorescence in Situ Hybridisation (FISH) analysis of interphase nuclei and other molecular methods of rapid aneuploidy diagnosis such as MLPA and BACs-on-Beads (BoBs), where MCC of samples from female pregnancies cannot be detected.
This paper offers solid advice for laboratory professionals on using a QF-PCR-based approach to identify maternal cell contamination in uncultured AF and CV samples. Authored by Dr. Kathy Mann, the pioneer in prenatal QF-PCR analysis.
QF-PCR in Pregnancy Loss Analysis by Dr. Helen White
This paper provides insights for laboratory professionals on the advantages of using extended QF-PCR in the genetic analysis of pregnancy loss samples. The paper includes useful sections on: current technologies, QF-PCR analysis in routine practice, special considerations and more. Authored by Dr. Helen White, Senior Clinical Lecturer at the UK National Genetics Reference Laboratory (Wessex).
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We are specialists when it comes to diagnostic kits for complex DNA testing within oncology, reproductive health and hereditary diseases. Our products are used to guide targeted cancer therapies, to enable rapid prenatal diagnostics, as well as in a wide array of genetic tests. Routine diagnostic laboratories in more than 50 countries worldwide use our products.