Overcoming limitations in the detection of mixed chimerism

This paper by Dr. Dan Hauzenberger, Medical Director of the Section for Transplantation Immunology at Karolinska University Hospital, Sweden, highlights the importance of chimerism as a diagnostic tool for clinicians treating transplanted patients. Furthermore, the evolution of novel diagnostic tools for early detection of mixed chimerism is discussed. The recent development of NGS technology offers the possibility to analyze mixed chimerism with both sensitivity, as well as accurate and precise determination.

• Importance of detecting mixed chimerism.
• Methods for detection of mixed chimerism.
• Recent development of NGS technology and its possibilities.