---
title: A new NGS-based method for chimerism monitoring | Devyser Blog
description: Dr. Dan Hauzenberger at Sweden’s Karolinska University Hospital, on how new, highly sensitive and precise methods for mixed chimerism analytics are transforming post-transplant monitoring, enabling earlier detection and treatment to improve and save the lives of transplanted patients
---

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# [A new NGS-based method for chimerism monitoring | Devyser Blog](https://devyser.com/blog/a-new-ngs-based-method-for-chimerism-monitoring)

 Written by [Admin](https://devyser.com/blog/author/admin) | Jan 22, 2021 3:00:00 PM

Dr. Dan Hauzenberger, Medical Director, Section of Transplantation Immunology at Sweden’s Karolinska University Hospital, on how new, highly sensitive and precise methods for mixed chimerism analytics are transforming post-transplant monitoring, enabling earlier detection and treatment to improve and save the lives of transplanted patients. He also discusses why Karolinska decided to replace their STR-PCR and qPCR methods with a single, NGS-based method and the benefits achieved by doing so.

### Case Study Preview

For at least 15 years Karolinska’s Transplantation Immunology Laboratory has used a combination of STR-PCR and qPCR, two well-established methods for detecting mixed chimerism. Both have clear advantages and disadvantages. The STR-PCR-based methods generally show high precision but a restricted limit of detection (LOD); qPCR on the other hand exhibits a high sensitivity but poor precision, especially at higher levels of mixed chimerism.

"While I am delighted about the increased efficiency and cost reductions we’ve achieved in the laboratory, I am even more excited about the sharper diagnostic tools we can now offer clinicians."  
Dr. Dan Hauzenberger, M.D., Ph.D. Karolinska University Hospital, Stockholm, Sweden

[View full post](https://devyser.com/blog/a-new-ngs-based-method-for-chimerism-monitoring)

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