QF-PCR and maternal cell contamination by Dr. Kathy Mann

QF-PCR and MCC

The ability of QF-PCR to identify Maternal Cell Contamination (MCC) in uncultured Amniotic Fluid (AF) and Chorionic Villi (CV) offers a real benefit over both Fluorescence in Situ Hybridisation (FISH) analysis of interphase nuclei and other molecular methods of rapid aneuploidy diagnosis such as MLPA and BACs-on-Beads (BoBs), where MCC of samples from female pregnancies cannot be detected.

This paper offers solid advice for laboratory professionals on using a QF-PCR-based approach to identify maternal cell contamination in uncultured AF and CV samples. Authored by Dr. Kathy Mann, the pioneer in prenatal QF-PCR analysis.