Detecting mosaicism with QF-PCR by Dr. Kathy Mann

QF-PCR Mosaicism

Given the significance of mosaicism to prenatal diagnosis, it is clear that any technique used in the diagnosis of aneuploidy in prenatal samples should be able to detect aneuploid mosaicism. With these criteria in mind, the use of quantitative fluorescence-PCR (QF-PCR) analysis is suitable (see Expert Review 01: An introduction to QF-PCR).

This paper provides straightforward advice for laboratory professionals on how to use QF-PCR to identify mosaicism in prenatal diagnostics. Authored by Dr. Kathy Mann, the pioneer in prenatal QF-PCR analysis, it includes useful sections on: origin, detection and incidence of mosaicism and more.